Fig 1.
Agarose gel electrophoresis of HSP70-I-3′-UTR PCR products of 12 Leishmania species.
M = Molecular markers and N = Negative control.
Table 1.
In silico prediction of the HSP70-I-3′-UTR-BsuRI restriction fragments and fragment sizes in PCR-RFLP.
Fig 2.
Agarose gel electrophoresis of HSP70-I-3′-UTR PCR products of DNA extracted from clinical samples.
A. HSP70-I-3′-UTR PCR products B. PCR products amplified using UNFOR403 and UNREV1025 primers. M = Molecular markers and N = Negative control.
Fig 3.
Microsatellite distribution in 3’-UTR of HSP70-I genes of L. martiniquensis, L. orientalis, and L. mexicana.
The repeated motifs correspond to those found in the sense strand. Note that the drawing scale is not proportional for the different Leishmania species.
Fig 4.
PCR-RFLP analyses of HSP70-I-3′-UTR fragments of 12 Leishmania species.
PCR amplification was performed with the HSP70-I-3′-UTR-specific primers, and PCR products were digested with BsuRI. M = Molecular markers and N = Negative control.
Fig 5.
Agarose gel electrophoresis of PCR products of L. martiniquensis and L. orientalis.
A. HSP70-I-3′-UTR PCR products B. SSU-rRNA PCR products C. ITS1-rRNA PCR products. Human blood DNA at 19.8 ng per reaction was used as a background control. N = negative control and lanes 1–10 indicate amounts of 100,000; 10,000; 1,000; 100; 10; 1; 0.1; 0.01; 0.001; and 0.0001 pg of Leishmania DNA in each reaction, respectively.