Table 1.
PBP homologs in B. pseudomallei 1026b.
Fig 1.
DLST scheme based on 11 nucleotides in two genes (I0276 and II1314) encoding putative PBP-3s.
Nucleotide positions are based on sequence alignment to the B. pseudomallei 1026b reference strain. Nucleotides with phylogeographic utility (orange font) and utility for differentiating closely related Burkholderia species (purple font) are shown. PBP conserved domains (blue bubbles), amino acid position of domains (blue font), active site residues and positions (red font).
Fig 2.
DLST SNP-based phylogeographic tree for the initial set of B. pseudomallei (n = 101) and B. mallei (n = 26).
Each branch represents isolates with a distinct 11-nucleotide SNP signature determined by DLST. B. pseudomallei strains are color-coded by geographic origin and SNPs used to differentiate B. mallei strains are shown in dark yellow.
Fig 3.
DLST SNP-based phylogeographic tree for the expansive set of B. pseudomallei (n = 1,442, sequence types 1 to 31) and B. mallei (n = 81, sequence type 32).
B. pseudomallei strains are color-coded by geographic origin. Western Hemisphere (WH), asterisk indicates WH and European countries Mexico, Ecuador, Venezuela, Czech Republic, and Switzerland.
Table 2.
Single amino acid polymorphisms found in PBP transpeptidase domains of B. pseudomallei Bp1651.
Table 3.
PBP SNPs with utility for differentiation of Burkholderia species.
Table 4.
Dual-locus sequence typing scheme.