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Fig 1.

Diagram flow of recruitment of 5 to 15 years old participants and over 15 years old participants and their sample collection and analysis.

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Table 1.

Description of study participants’ characteristics by frequency and percentage, n (%).

Water and sanitation are defined according UNICEF Joint Monitoring Programme [33].

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Table 2.

Number of participants infected by each soil-transmitted helminth detected by Telemann in one or two stool samples, single and duplicate Kato-Katz in one stool sample, single and duplicate Kato-Katz in two stool samples, multiplex qPCR and by a composite reference standard (CRS, positive for at least one diagnostic technique) per each age group.

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Fig 2.

Estimated sensitivity and 95% confidence intervals [95% CI] of Telemann in one or two stool samples, single and duplicate Kato-Katz in one stool sample, single and duplicate Kato-Katz in two stool samples, multiplex qPCR compared to the composite reference standard (CRS) per A. lumbricoides, T. trichiura and hookworm.

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Table 3.

Number and percentage of participants with a low, moderate or high intensity of any STH infection detected by single Kato-Katz from one stool; according to WHO. [34].

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Fig 3.

Agreement between qPCR Ct value with fecal egg count logarithm of the four quantitative microscopic methods (single and duplicate Kato-Katz in one stool sample, and single and duplicate Kato-Katz in two stool samples) for A. lumbricoides, T. trichiura and hookworm. In each graph, the concordance correlation coefficient (ρ) and p-value are provided.

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Fig 4.

District map of the estimated prevalence of at least one STH infection calculated with a generalized linear model assuming a binomial distribution for Telemann in one and two stools, single and duplicate Kato-Katz in one stool, single and duplicate Kato-Katz in two stools and qPCR in one stool.

Base layer map obtained in https://data.humdata.org/dataset/mozambique-administrative-levels-0-3.

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