Fig 1.
Map of Egypt showing the different provinces where the blood samples from different animal hosts were collected for our study.
https://en.wikipedia.org/wiki/Governorates_of_Egypt and the picture has CC BY-SA 3.0.
Table 1.
The information data of collected samples.
Table 2.
Primers and probes used for qPCR, Standard PCR and sequencing in this study.
Table 3.
The prevalence of pathogens in animals by PCR.
Fig 2.
18S rRNA based phylogenetic analysis of genotypes identified in this study.
Phylogenetic tree highlighting the position of Theileria sp. and Babesia sp. in the present study (Bold) related to other Theileria sp. and Babesia sp. available in GenBank. The sequence of 18S rRNA were aligned using CLUSTAL W and phylogenetic inferences were constructed in MEGA X using Maximum Likelihood based on Tamura-Nei Model for nucleotide sequences with 500 bootstrap replicates. There was a total of 1066 positions in the final dataset. The scale bar represents a 2% nucleotide sequence divergence.
Fig 3.
23S rRNA based phylogenetic analysis of genotypes identified in this study.
Phylogenetic tree highlighting the position of Anaplasma sp. in the present study (Bold) related to other Anaplasma sp. and Ehrlichia sp. available in GenBank. The sequence of 23S rRNA were aligned using CLUSTAL W and phylogenetic inferences were constructed in MEGA X using Maximum Likelihood based on Tamura-Nei Model for nucleotide sequences with 500 bootstrap replicates. There was a total of 432 positions in the final dataset. The scale bar represents a 5% nucleotide sequence divergence.
Fig 4.
rpoB gene based phylogenetic analysis of genotypes identified in this study.
Phylogenetic tree highlighting the position of Anaplasma sp. in the present study (Bold) related to other Anaplasma sp. and Ehrichia sp. available in GenBank. The sequence of rpoB gene were aligned using CLUSTAL W and phylogenetic inferences were constructed in MEGA X using Maximum Likelihood based on Tamura-Nei Model for nucleotide sequences with 500 bootstrap replicates. There was a total of 534 positions in the final dataset. The scale bar represents a 10% nucleotide sequence divergence.
Fig 5.
gltA gene based phylogenetic analysis of genotypes identified in this study.
Phylogenetic tree highlighting the position of Rickettsia sp. in the present study (Bold) related to other Rickettsia sp. available in GenBank. The sequence of gltA gene were aligned using CLUSTAL W and phylogenetic inferences were constructed in MEGA X using Maximum Likelihood based on Tamura-Nei Model for nucleotide sequences with 500 bootstrap replicates. There was a total of 728 positions in the final dataset. The scale bar represents a 2% nucleotide sequence divergence.
Fig 6.
16S rRNA based phylogenetic analysis of genotypes identified in this study.
Phylogenetic tree highlighting the position of Borrelia sp. in the present study (Bold) related to other Borrelia sp. available in GenBank. The sequence of 16S rRNA were aligned using CLUSTAL W and phylogenetic inferences were constructed in MEGA X using Maximum Likelihood based on Tamura-Nei Model for nucleotide sequences with 500 bootstrap replicates. There was a total of 1142 positions in the final dataset. The scale bar represents a 5% nucleotide sequence divergence.
Fig 7.
18S rRNA based phylogenetic analysis of genotypes identified in this study.
Phylogenetic tree highlighting the position of Filaria sp. in the present study (Bold) related to other Filaria sp. available in GenBank. The sequence of 18S rRNA were aligned using CLUSTAL W and phylogenetic inferences were constructed in MEGA X using Maximum Likelihood based on Tamura-Nei Model for nucleotide sequences with 500 bootstrap replicates. There was a total of 1110 positions in the final dataset. The scale bar represents a 10% nucleotide sequence divergence.