Fig 1.
(A) represents Thailand and the provinces of Roi Et and Khon Kaen. (B) shows the location of the 18 clusters numbered from 4001 to 4018 in the city of Khon Kaen (KK Mueang District). (C) shows the location of the 18 clusters numbered from 4501 to 4518 in the city of Roi Et (RE Mueang District). Map of study sites was built using QGis 3.10 software and shapefiles were obtained from the Humanitarian Data Exchange project [49] under the Creative Commons Attribution International 4.0 license (CC BY 4.0).
Fig 2.
RT-PCR: Reverse Transcriptase Polymerase Chain Reaction.
Table 1.
Population description and immunological status to Nterm-34 kDa salivary peptide.
Table 2.
Individual participant characteristics, behavior and occupation.
(NA: Not available).
Table 3.
Entomological collection data and indices at household and cluster level.
Fig 3.
Seasonal variations of the human IgG response to Aedes Nterm-34kDa salivary biomarker and the adult density Aedes Index (AIc), between September 2017 and April 2019 in Khon Kaen (A) and Roi Et (B) northeastern Thailand. The dot plots represent the individual IgG immune response to the Aedes salivary peptide Nterm-34 kDa (ΔOD). The red diamonds represent the median response during each survey. The solid red lines represent the means and the grey shaded areas represent the confidence interval of the IgG response to the salivary biomarker. The red dashed horizontal lines represent the specific immune threshold TR. The solid blue lines represent the means and the grey shaded areas represent the 95% confidence interval respectively, for the AIc at the cluster level.
Fig 4.
Multivariate analysis of MEI, human immune response to the Nterm-34 kDa salivary.
(A) Adult Aedes indoors index only multivariate model. (B) Adult Aedes multivariate model.
Table 4.
Multivariate analysis of MEI, human immune response to Nterm-34 kDa salivary peptide.
Table 5.
Multivariate mixed linear model of human immune response to Nterm-34kDa Aedes salivary peptide or MEI and the presence of DENV infected Aedes in the cluster.