Fig 1.
Photograph of a FeverDisk cartridge.
All reagents are pre-stored for fully automated nucleic acid analysis. The sample inlet is sealed with a pre-mounted sealing foil.
Fig 2.
Photograph of the FeverDisk processing device.
Dr. Cheikh Fall, co-author from the Institut Pasteur de Dakar, prepares a FeverDisk for measurement.
Table 1.
Overview of results from the test series in Senegal where 29 FeverDisks were used.
The reference methods as well as the FeverDisk results are shown.
Fig 3.
Detection of CHIKV and DENV4 co-infection in sample 274755.
The FeverDisk showed a true positive DENV4 amplification in addition to the CHIKV-expected amplification.
Fig 4.
Detection of CHIKV and DENV2 co-infection in sample 274754.
The FeverDisk showed a true positive DENV2 amplification in addition to the CHIKV-expected amplification.
Fig 5.
Detection of DENV and determination of initially unknown serotype.
Sample 267197 is shown here; sample 267175 showed similar results.
Table 2.
Overview of results from the test series in Sudan where 9 FeverDisks were used.
The reference methods as well as the FeverDisk results are shown.
Fig 6.
Detection of malaria on a fresh whole blood sample collected in real time.
Positive signal for Pf and Pspp was confirmed as true positive with microscopy blood smear test.
Table 3.
Bacterial concentrations tested in the FeverDisk to calculate the limit of detection and the ratio of positive results within the total repetitions assayed.
Table 4.
FeverDisk false positive results.
The results are considered as such, when the Tp values are higher than those that were specified, i.e. Tp > 44 min for the Plasmodium assays; Tp > 60 min for the bacterial assays; Tp > 64 min for the CHIKV, DENV viral assays.