Table 1.
Glycans bound by M. ulcerans cells and MUL_3720 protein in glycan array analysis.
Red labelling indicates binding as determined by interaction above background in three replicate array experiments. A positive fluorescent value is defined as any value above the average background fluorescence of 20 negative control spots + 3 standard deviations. Data are presented as fold-above this background value, which were 2706±372 RFU for M. ulcerans cells and 1829±140 RFU for the MUL_3720 studies. Only glycans that showed binding are shown; see S1 Data for full array results and list of all the glycans present on the array.
Table 2.
SPR results for the binding of M. ulcerans cells and MUL_3720 protein to selected glycans.
Shown are dissociation equilibrium constants (KD) of the interactions between free oligosaccharides and captured M. ulcerans cells and MUL_3720 protein; NCDI: no concentration-dependent interaction detected up to a maximum concentration of 20 μM. See S2 Data for representative sensorgrams.
Fig 1.
SPR competition analysis of ΔUAα 1-3GalNAc-6S and keratin extract.
Values are the average maximum endpoint response units obtained individually and in competition at 10x KD. The two columns in orange represent the expected values for either a shared binding site (average signal for keratin and ΔUAα 1-3GalNAc-6S) or separate binding sites (additive signal for keratin and ΔUAα 1-3GalNAc-6S).