Table 1.
Metrics for de novo transcriptome assembly.
(SG0, salivary gland from unfed ticks; SG7 and SG14, salivary gland from ticks at 7 and 14 days after a blood meal respectively).
Fig 1.
Ornithodoros moubata sialotranscriptome.
Classification of the annotated transcripts according to the molecular function (A) and biological process (B). Only categories represented by more than 400 genes are included in the pie charts. For each category, the number and percentage of genes are indicated.
Table 2.
Top 30 more represented biological pathways in the sialotranscriptome of O. moubata.
Fig 2.
Transcriptome differential expression patterns.
Scatterplots (one per each differential expression analysis) representing the log Fold Change (log2FC) against average of logCPM per each transcript across each pair of compared samples. Differentially expressed transcripts with FDR < 0.05 and log2FC ≥ and ≤ 1 are plotted in red. SG0, salivary glands from unfed ticks; SG7 and SG14, salivary glands from ticks at 7 and 14 days after feeding, respectively. vs, versus.
Fig 3.
Venn diagram showing the number of differentially expressed genes in each comparison.
SG0, salivary glands from unfed ticks; SG7 and SG14 salivary gland from ticks at 7 and 14 days after feeding, respectively. vs, versus.
Fig 4.
Top 20 GO terms of Biological Process differentially enriched in each comparison: SG7 vs SG0, SG14 vs SG0 and SG14 vs SG7.
SG0, salivary glands from unfed ticks; SG7 and SG14, salivary glands from ticks at 7 and 14 days after feeding, respectively. NumDEInCat, number of enriched sequences in each category. vs, versus.
Fig 5.
Top 20 Molecular Function GO terms of differentially enriched in each comparison: SG7 vs SG0, SG14 vs SG0 and SG14 vs SG7.
SG0, salivary glands from unfed ticks; SG7 and SG14, salivary glands from ticks at 7 and 14 days after feeding, respectively. NumDEInCat, number of enriched sequences in each category. vs, versus.
Table 3.
Metabolic pathways differentially enriched in each comparison: SG7 vs SG0, SG14 vs SG7 and SG14 vs SG0.
SG0, salivary glands from unfed ticks; SG7 and SG14, salivary glands from ticks at 7 and 14 days after feeding. Num DE seq in Pathway, number of enriched sequences in the pathway. Seqs in Pathway, number of sequences included in the pathway.
Fig 6.
Number of upregulated annotated transcripts in each functional group (green bars) and expression levels in RPKM in comparisons SG7 vs SGO and SG14 vs SG0 (red bars).
Percentages at the red bar ends represent the ratio between the expression level of each group/family and the total expression in RPKM of the whole annotated upregulated transcriptome at 7 days (A) and at 14 days (B). SG0, salivary glands from unfed ticks; SG7 and SG14, salivary glands from ticks at 7 and 14 days after feeding, respectively. vs, versus.
Table 4.
Lipocalins: transcripts annotated and differentially upregulated in the O. moubata sialotranscriptome 7 days after feeding (SG7 vs SG0).
The number of transcripts, the average expression level (RPKM) in each physiological condition (SG0, SG7) and the logFC value range reached by the upregulated transcripts are shown.
Table 5.
Metalloproteases: transcripts annotated and differentially upregulated in the O. moubata sialotranscriptome 7 days after feeding (SG7 vs SG0).
The number of transcripts, the average expression level (RPKM) in each physiological condition (SG0, SG7) and the logFC value range reached by the upregulated transcripts are shown.
Table 6.
Proteases: transcripts annotated and differentially upregulated in the O. moubata sialotranscriptome 7 days after feeding (SG7 vs SG0).
The number of transcripts, the average expression level (RPKM) in each physiological condition (SG0, SG7) and the logFC value range reached by the upregulated transcripts are shown.
Table 7.
Protease inhibitors: transcripts annotated and differentially upregulated in the O. moubata sialotranscriptome 7 days after feeding (SG7 vs SG0).
The number of transcripts, the average expression level (RPKM) in each physiological condition (SG0, SG7) and the logFC value range reached by the upregulated transcripts are shown.
Table 8.
Acid and basic tail proteins, vitellogenins, proteins with phospholipase A2 activity, a member of the 7DB family and proteins involved in immunity and defense.
Transcripts annotated and differentially upregulated in the O. moubata sialotranscriptome 7 days after feeding (SG7 vs SG0). The number of transcripts, the average expression level (RPKM) in each physiological condition (SG0, SG7) and the logFC value range reached by the upregulated transcripts are shown.