Fig 1.
Selection of new antibody pairs to NS1 protein of four DENV serotypes by the modified stNS1-ELISA.
A) Schematic diagram of stNS1-ELISA in the original format (left) and the modified format (right); CR = cross reactive, SS = serotype-specific. B) Serotype-specificity of 8 selected antibody pairs to NS1 from each DENV serotype. Each antibody pair is composed of capture MAb and biotinylated detection MAb (*). DENV1 NS1 (blue), DENV2 NS1 (red), DENV3 NS1 (green), DENV4 NS1 (pink). C) Comparison of OD readings of clinical samples obtained from the original format (orange) and the modified format (blue).
Table 1.
Preliminary testing of the modified stNS1 ELISA compared with the original format in clinical samples.
Table 2.
Comparison of the antibody pairs and detection limit between the original and the modified stNS1-ELISA.
Table 3.
Performance evaluation of the modified stNS1-ELISA vs. RT-PCR in clinical specimens.
Fig 2.
(A) Schematic diagram of the modified stNS1-ELISA (plus) containing five antibody pairs, four of which were serotype-specific and the other was DENV1/3 sub-complex. (B) Reactivity of antibody pairs to purified NS1 of each dengue serotype.
Table 4.
Performance evaluation of the modified stNS1-ELISA (plus) vs. RT-PCR in clinical specimens.
Table 5.
Performance of dengue NS1-RDT vs. standard RT-PCR.
Table 6.
Comparison of overall performance of the modified stNS1-ELISA, the modified stNS1-ELISA (plus) and NS1-RDT.