Table 1.
Genes and primers used in this study.
Nested PCR and qPCR assays were conducted for detection, titration, and typing of Coxiella burnetii.
Fig 1.
Coxiella burnetii according to sex and organ in Ornithodoros moubata ticks.
Infection densities in males (n = 4, orange circles and boxplots) and females (n = 4, green circles and boxplots) were quantified by using the ratio of bacterial egfp gene copies per tick OmAct2 gene copy and represented as the logarithm values (× + 0.001).
Fig 2.
A. eGFP-expressing C. burnetii amplified from ACCM2 culture broth inoculated with samples from the second blood meal on which infected ticks fed. B. U2OS cells challenged with eGFP-expressing C. burnetii amplified as described in A were fixed and processed for immunofluorescence 6 days post-infection. Scale bars are 20 μm (A) and 10 μm (B).