Fig 1.
10% native polyacrylamide gel electrophoresis demonstrates the RFLP patterns of kdr T917I genotypes.
Lane 1 Undigested PCR products, lane 2, 6 and 9 are representative of the homozygous wild type (SS). Lane 3–5 heterozygous genotype (RS) have three bands. Lane 7 and 8 are representative of the homozygous mutation (RR) presented with two bands. Lane M; a 50 bp DNA marker.
Fig 2.
Distribution of kdr T917I genotypes in human head lice collected from 6 geographical regions in the 15 provinces of Thailand.
(The figure modified from the public domain (https://landsatlook.usgs.gov/)).
Table 1.
Frequency of kdr T917I genotype in human head lice collected from different geographical regions of Thailand.
Fig 3.
An alignment of nucleotide sequences (A) and amino acid sequences (B) of the α subunit of VSSC gene of human head lice collected from Thailand. The sequence showed the position of the kdr T917I mutation as well as the position of L920F mutation indicated by a vertical column.
Fig 4.
Chromatograms of heterozygous genotype (RS) from direct sequencing (A) equal signal of the nucleotide C and T at T917I and L920F are observed. Chromatograms of clones of the PCR products of the RS clearly demonstrate nucleotide C (B) or nucleotide T (C) signals, showing the kdr T917I and L920F of wild type and mutations, respectively.