Fig 1.
A. Calibration curve for the ABA-1 coproantigen ELISA. Yellow area indicates the dynamic range of the assay, left vertical dotted line corresponds to the LLOQ (0.137 ng/mL), right vertical dotted line to the ULOQ (11.1 ng/mL), the horizontal dotted line corresponds to the background signal detected in blanks. B. Analysis of a serial dilution of pseudocoelomic fluid (blue circles) and an adult worms extract (red circles) on the ABA-1 ELISA. C. ABA-1 coproantigen ELISA using stool extracts with and without bead beating indicates that a cell destruction step is needed to release ABA-1 in the stool supernatant.
Fig 2.
A. Assessment of ABA-1 coproantigen levels in stool samples from a cohort of 474 subjects collected in Kenya, stratified according to their A. lumbricoides qPCR result. Open circles indicate subjects with M&HI infection. B. Correlation between ABA-1 coproantigen levels and A. lumbricoides DNA detection in stool collected in Kenya (expressed in A. lumbricoides copies/reaction). Based on the linear regression, a cut-off of 18.4 ng/g stool was defined to identify subjects with M&HI infection. Moderate infection was defined as >700 cps/rxn (see Materials and Methods). C. Effect of treatment with albendazole on the presence of ABA-1 in stool. Stool samples were collected before (Day 0) and at different timepoints (6, 12 and 24 days) after treatment with albendazole.
Fig 3.
Quantification of fecal egg count (A) and ABA-1 in stool (B) from A. suum infected pigs: control group (green), low (red) and high (blue) trickle infected pigs. Stool samples were collected before infection (Day 0) and at different timepoints during trickle infection: 14 days, 28 days, 42 days and 56 days.