Fig 1.
Injection of Loa loa mf into C. silacea.
Here, 0.5 ml insulin syringe containing Loa loa mf is introduced to a ventral positioned Chrysops fly held with a pair of forceps.
Table 1.
C. silacea survival rate and number of L3 of L. loa recovered 14 days following intrathoracic injection of mf.
Table 2.
Average L3 recovered from different parts of C. silacea injected intrathoracically with 50 and 100 mf of L. loa at day 14 p.i.
Fig 2.
Survival and motility monitoring of L. loa L3 derived from mf injections of C. silacea, in mammalian in vitro culture conditions.
Percentage survival curve (A) and motility curve (B) of Loa loa larvae during 30 days of culture. Plotted is the average (mean) response. Bars indicate standard deviations (SD).
Fig 3.
Representative image of an in vitro L. loa L4 larvae and cuticle cast.
Image taken by inverted tissue culture microscope. Arrows: red = fully moulted Loa L4; black = Cast cuticle from moulted Loa L3. Scale bar 100μM.
Table 3.
in vitro culture moulting success of L. loa L3 derived from mf injection of C. silacea.
Fig 4.
Loa loa adult morphology 62 days post-inoculation.
(A and B) Head of young adult female stage (anterior region); Mo–mouth opening; Eo—esophagus. (c): Vulva [Vu] of mature young adult female (lateral view). (D): Open vulva, intestine, uterus of female young adult worm with no mf present (indicating that the worm is not fully developed [lateral view]). (E and F): Posterior extremity of matured young adult female worm (E; lateral view [An–anus] and F; ventral view) with caudal papillae [Pa]. (G and H) Caudal region (lateral view) of Loa loa young adult male with caudal papillae (G) and spicule (H). [A, D, E, G viewed under x10 objective, B, C viewed under x40 objective F, H viewed under x100 objective].
Table 4.
Recovery of adult L. loa from RAG2-/-IL-2γc-/- mice infected with L3 derived from experimental injections of C. silacea.