Fig 1.
Multilocus conventional PCR for Trypanosoma cruzi genotyping in clinical samples [19].
The flowchart indicates the gene targets and the sizes of the PCR products expected for the DTUs.
Table 1.
General information of patients involved in the study and criteria for genotyping of Trypanosoma cruzi.
Fig 2.
Parasitic load of Latin American immigrants with chronic ChD residing in Barcelona. Distribution according to (A) the country of origin; (B) the indeterminate and cardiac forms. The horizontal lines represent the mean values of the parasite load.
Fig 3.
Representative gels of amplified gene products to define Trypanosoma cruzi DTUs.
Genes: (A) the SL-IRac; (B) the SLIR I and II; (C) the 24Sα rDNA; (D) the A10. (MW—Molecular Weight marker; NC—Negative control; Positive controls, amplified products of reference strains: TcI, Colombiana; TcII, Y; TcIII, X109/2; TcIV, CANIII cl1; TcV, Bug2148 cl1; TcVI, CL Brener. The numbers indicate the code of the sample (see Table 1). Patient’s sample and infecting DTU: 1, TcV; 19, TcVI; 56, TcII/TcVI; 90, TcII/TcVI; 2, TcII/TcVI; 26, TcII/TcV/TcVI; 82, TcII/TcV/TcVI.
Table 2.
Genotyping of Trypanosoma cruzi from peripheral blood of chronic chagasic immigrant patients.