Fig 1.
Map showing area of sample collection (Anambra, Enugu and Ebonyi State).
Map created using ArcView 8.0 software.*Arrow showing the distance from South East region to the rabies diagnostic reference laboratory.
Fig 2.
Flow diagram of specimens’ collection and diagnosis.
Table 1.
Oligonucleotide primers and probe sequences for the “one-step” real-time qRT-PCR amplification of the nucleoprotein gene of the lyssavirus genome.
Table 2.
The Oligonucleotide primer sequences used in the study showing the annealing positions and their nucleotide sequences [39].
Table 3.
Estimated “total cost per sample” for both the direct fluorescent antibody (DFA) and direct, rapid immunohistochemical test (dRIT) assays.
Table 4.
Diagnostic overview of the brain tissue samples tested with the direct fluorescent antibody test in Nigeria, disaggregated by State and sample cohort.
Table 5.
Neuronal tissue sample cohort from Nigeria depicting the initial diagnostic results from the NVRI in Nigeria and their diagnostic testing at the laboratories in South Africa.
Table 6.
Diagnostic sensitivity and specificity of the three OIE recommended diagnostic assays applied to a cohort of samples collected from dog meat markets.
Fig 3.
Hemi-nested PCR showing amplification products of approximately 606 bp following agarose gel electrophoresis.
*M– 100 bp Molecular weight marker (Promega); C- Negative control; 811/97—Positive control.
Fig 4.
Molecular phylogenetic analysis by neighbour joining method showing the five samples (green dots) with discordant DFA, dRIT and hn-qPCR results forming part of the canine variant of RABV that clustered with other Nigerian RABVs.