Fig 1.
A. Geographic localization of Montes Claros city (in red) in Minas Gerais state, Brazil; B. Geographic localization of Independência neighborhood (in red) in Montes Claros, Minas Gerais, Brazil. Created by the authors using Adobe Photoshop CS4 software.
Fig 2.
Experimental dog during xenodiagnosis.
A. Lu. longipalpis females were placed in a plastic box with an open top covered by a fine nylon mesh and the box was fixed on the inner skin of the dog's ear. B. The whole set was covered with a piece of black tissue for 30 minutes. C. Lu. longipalpis specimens were collected for molecular analysis. Engorged females can be noticed near the nylon mesh.
Table 1.
Canine census surveys for canine visceral leishmaniasis performed semiannually in an area of recent moderate epidemiological risk of transmission of visceral leishmaniasis.
The study was performed in Independência neighborhood in Montes Claros (Minas Gerais state, Brazil). Aug/2015 to Feb/2017.
Table 2.
Seropositive dogs recommended to euthanasia, in accordance with the Visceral Leishmaniasis Surveillance and Control Program (VLSCP) guidelines of the Brazilian Ministry of Health.
The study was performed in an area of recent moderate epidemiological risk of transmission (ETR). Montes Claros (Minas Gerais state, Brazil). Aug/2015 to Feb/2017.
Table 3.
Follow-up of dogs with divergent serological statuses for canine visceral leishmaniasis (CVL) in an area of recent moderate epidemiological risk of transmission (ETR).
New serological tests (DPP and ELISA) were performed every three months after the first diagnosis, during up to nine months. Montes Claros (Minas Gerais state, Brazil). Aug/2015 to Feb/2017.
Table 4.
Xenodiagnosis results of dogs serologically tested for canine visceral leishmaniasis in Montes Claros (Minas Gerais state, Brazil) according to the current protocol adopted by the Brazilian Ministry of Health.
Dogs’ IDs are represented by the last five digits of the microchip. Abbreviation: gz = grey zone in ELISA (see Material and Methods). Blood sample collection: A. CCS4 (Feb/2017); B. Immediately before xenodiagnosis (Mar/2017). Study period: Aug/2015 to Feb/2017.