Fig 1.
Use of ZFN results in efficient editing of the AaeCad gene.
A. A ZFN construct with zinc finger nuclease binding site (in black, uppercase) and target site (in red, lower case). B. After G1 mosquitoes were genotyped and the generated data was analyzed and plotted. Profiling of the deletion length and the mutation rate is shown for a representative group of G1 mosquitoes. C. A chromatogram of a ZFN-targeted AaeCad fragment from wild-type Orlando strain mosquitoes and heterozygous mutant with 4nt-deletion. For ZFN-targeted AaeCad fragment from the heterozygous mutant, the overlapping chromatography peaks are displayed after the 4nt deletion.
Fig 2.
TALEN mediated knockout of the AaeCad gene.
A. In this TALEN design schematics shows AaeCad gene sequence targeted by TALEN. B. G2 mosquitoes were genotyped by native heteroduplex mobility assay (HMA). Four out of 117 mosquito groups analyzed had 3nt, 4nt, 6nt or 3nt & 4nt deletions, respectively. Lane M, DNA marker; Lane 1, sample mosquito #31M with 4nt indel; Lane 2, sample #32M with 3nt and 4nt indel; Lane 3, sample #41 with 3nt indel; Lane 4, sample #70F with 6nt indel; Lane 5, sample #24F with no indel. C. PCR product sequencing from a heterozygous mutant displayed overlapping chromatogram peaks, but those from wild-type Orlando strain mosquito showed single chromatogram peaks.
Fig 3.
CRISPR-Cas9 mediated EGFP tagging at the 3’ end of the last coding exon of AaeCad gene results in an in frame fusion.
A. sgRNA (in red) and PAM sequences (in blue) were designed before the stop codon (in purple) on the last exon of AaeCad gene. B. This donor plasmid for homologous recombination (HR) contains 1000bp of left arm, 720 bp of EGFP ORF and 1000bp of right arm. To prevent Cas9 cleavage on the left arm in the plasmid, a silent mutation (CGG → CTG) was introduced into the PAM sequence on the left arm. C. When the homologous recombination occurred, the EGFP was introduced immediately after the AaeCad gene with the aid of the left and right arms. D. The sequencing results of gDNA showed the AaeCad gene had been successfully tagged with EGFP and no mutation was introduced into the AaeCad gene except for the silent mutation that was intentionally introduced.
Table 1.
Use of either ZFN or TALEN led to knockout of the Aedes cadherin gene, but ZFN is far more efficient.
Table 2.
CRISPR-Cas9 mediated homologous recombination to generate an EGFP tagged cadherin gene is more efficient with Rad51.
Fig 4.
Visualization of AaeCad-EGFP in whole mounts of dissected guts from larva, pupa and adult male and female homozygous Aedes.
A. AaeCad protein localization in early 4th instar larval gut. Expression is observed primarily in the gastric caecae and the posterior midgut. B. AaeCad protein expression in pupal gut. C. AaeCad protein localization in adult male gut; D. AaeCad protein localization in adult female gut. Expression in both the male and female was observed primarily in the foregut. Since the tissues were in PBS buffer the samples move ever so slightly under the different filters, preventing use of the merge function. Bar: 50μm.
Fig 5.
AaeCad protein is localized primarily in gastric caecae and posterior midgut of early 4th instar larvae.
A. Whole mount images under low magnification showed that strong AaeCad-EGFP expression in the gastric caecae (GC) and posterior midgut cell membrane. B. Cross section images showed high AaeCad-EGFP expression in the gastric caecae (GC) and posterior midgut and low expression in the anterior midgut. In the gastric caecae and midgut, EGFP-tagged AaeCad is specifically expressed in the epithelial cell membrane. DAPI stains the nucleus. All images were collected using an SP5 Inverted confocal microscope. Bar: 50μm.
Fig 6.
An anti-AaeCad polyclonal detects the EGFP-tagged AaeCad.
Anti-AaeCad polyclonal antibody could detect the EGFP-tagged AaeCad in gastric caecae (A) and posterior midgut (E), but not in the anterior midgut (C). Whereas, when only the Alexa 647-labeled antibody was used, no fluorescence was detected in the gastric caecae (B), anterior midgut (D) and posterior midgut (F). Bar: 50μm, but is 25μm in panel E.
Fig 7.
EGFP-tagged AaeCad co-localizes in the larval gut cells with the Cry11Aa toxin.
The Cry11A toxin bound to the AaeCad-EGFP in the gastric caecae (A) and posterior midgut (E), but not in the anterior midgut (C). However, when the Cry11A toxin was absent, the fluorescence could not be observed in the gastric caecae (B), anterior midgut (D) and posterior midgut (F). Bar: 50μm, but is 25μm in panels E and F.
Fig 8.
Larval exposure to low level Cry11A toxin concentrations disrupts mosquito midgut cells and causes these cells to shed its cell membrane.
The larval midgut cell morphology was observed under a confocal microscope after the mosquitos were treated at the LC10 dose for 1 hr (A), 4 hr (B), 8 hr (C) and 16 hr (D). Changes in cell conformation were observed with Armadillo staining in cadherin-EGFP mosquitoes (E, F and G) at 18h. The same image is observed with DAPI, EFGP (cadherin) and Alexa 555 (Amardillo). Significant AaeCad-EGFP and Amardillo signaling is also observed in the intracellular compartments. Bar: 50μm.