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Fig 1.

Locations of the mosquito collection sites.

Source: MALVEC Project 2016, Institut Pasteur du Laos—IRD, SavGIS software.

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Table 1.

List of the Aedes aegypti populations collected from the north to the south and their GPS coordinates.

Population abbreviation names are used throughout the text.

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Table 2.

Resistance status of Aedes aegypti larvae (reference and field strains) against temephos, deltamethrin, permethrin, and DDT.

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Fig 2.

Mortality rates and 95% confidence intervals in WHO tube tests of wild adult Aedes aegypti populations from Laos.

USDA: susceptible reference strain.

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Table 3.

Insecticidal activity of temephos against Aedes aegypti larvae (reference strain and field populations) with and without inhibitors.

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Fig 3.

Mortality (%) and standard deviation of adult Aedes aegypti after exposure to permethrin (0.25%) and malathion (0.8%) with and without inhibitors (DEF: 8%, DEM: 8%, and PBO: 4%).

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Fig 4.

Activity of detoxification enzymes and 95% Confidence Interval in field caught Aedes aegypti populations in comparison to the susceptible reference strain (USDA): cytochrome P450 monooxygenases (P450s; A), Glutathione-S transferases (GSTs; B), Esterases (α and β-CCEs; C and D). Sample sizes are 47 specimens/population. An asterisk (*) denotes significantly higher levels of detoxifying enzyme compared to the susceptible reference strain USDA.

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Fig 5.

Haplotypes frequencies of V1016G and F1534C kdr mutations in Aedes aegypti populations from Laos.

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Table 4.

Genotype distribution and allelic frequency of the V1016G and F1534C kdr mutations.

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Fig 6.

Significant positive correlations identified CNV of detoxification genes and population resistance levels.

Resistance levels are expressed as RR50 for larvae and % survival to WHO insecticide test for adults.

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