Table 1.
Seroprevalence of bitten dogs by ELISA and predictive values of the rSP03B sero-strip per region.
Table 2.
Intraclass Correlation Coefficients for inter-person reliability.
Fig 1.
Graphical representation of results obtained per region.
(A) Seroprevalence (%) calculated based on the SGH-ELISA results are plotted per region. (B) Results obtained by the rSP03B sero-strip are visualized per region and plotted according to the different categories of the test, starting from (0) a negative test result; (1) a very faint signal; (2) a low positive signal; (3) a positive but less intense signal than the control band and (4) a strong positive signal, as intense as the control band. The size of the plotted circle represents the proportion of dogs classified per category.
Table 3.
Comparison of the rSP03B sero-strip with SGH-ELISA per region.
Fig 2.
Graphical representation of results obtained with the rSP03B sero-strip during longitudinal sampling of dogs.
Results obtained by the rSP03B sero-strip are visualized per month and plotted according to the different categories of the test to show the seasonal fluctuation of the proportion of bitten dogs. Category (0) represents a negative test result; (1) a very faint signal; (2) a low positive signal; (3) a positive but less intense signal than the control band and (4) a strong positive signal, as intense as the control band. The size of the plotted circle represents the proportion of dogs classified per category.
Fig 3.
Results of a Western Blot performed with the SGH and the rSP03B protein of P. perniciosus and sera samples of hosts experimentally bitten by various hematophagous arthropods including P. papatasi, P. sergenti, P. tobbi, C. felis, I. ricinus, and Cu. molestus. Sera from non-bitten mice were used as negative control. The location of the rSP03B protein is indicated with a star sign. (-), negative control sera; STD, Standard; AB, Amidoblack stain.