Table 1.
Primers and probes used for the qPCR assays.
Fig 1.
The sensitivity and DNA concentration across four DNA extraction protocols for Trichuris and Necator.
The left panel represents the sensitivity (sen) and geometric mean of DNA concentration expressed as genome equivalents per ml of DNA extract (mean; GE/ml) for 20 Necator americanus samples preserved in 96% ethanol and extracted by four DNA extraction protocols. The DNA extraction protocols include the QIAamp DNA Stool Mini kit without (SK) and with bead beating (SK + BB), DNeasy Blood & Tissue kit without (TK) and without bead beating (TK + BB). The right panel represents the same parameters across 36 Trichuris trichiura samples preserved in 96% ethanol. Each line represents a sample.
Table 2.
The sensitivity across four DNA extraction protocols for different levels of Trichuris and Necator infections.
The intensity of infection was classified as low (T. trichiura: fecal egg count (FEC) <1,000 eggs per gram of stool (EPG); N. americanus: FEC <2,000 EPG) and as moderate-to-heavy (T. trichiura: FEC ≥1,000 EPG; N. americanus: FEC ≥2,000 EPG). The zero FECs, represent subjects for which no eggs were found applying McMaster.
Fig 2.
The sensitivity and DNA concentration across three preservatives and three preservation times.
The line graphs represent the sequential differences in sensitivity (Sen) and geometric mean (mean) of DNA concentration expressed genome equivalents/ml (GE/ml) of DNA over time (65, 125 and 425 days) for 17 Ascaris lumbricoides, 19 Trichuris trichiura and 7 Necator americanus samples stored in three preservatives. The preservatives include 96% ethanol (left plot), 5% potassium dichromate (middle graph) and RNAlater (right graph). Each line represents a sample.