Table 1.
Species of non-human primates kept at FZB-BH and included at present study.
Table 2.
Serologically positive non-human primates for leishmaniasis with three different serological assays.
Legend: ID: individual identification of each animal. NA: not applicable.
Fig 1.
Transmission of Leishmania infantum from non-human primates to Lutzomyia longipalpis.
Ratio of positive and negative sand flies (n = 10) exposed to each of the positive animals, and tested for detection L. infantum DNA by qPCR.
Fig 2.
Quantitative analysis of Leishmania infantum in Lutzomyia longipalpis exposed to non-human primates.
Relative quantification of L. infantum promastigotes in each individual positive sand fly according to the non-human primate species. Symbols refer to individual animals: open symbols indicate negative sand flies, and solid symbols indicate infected sand flies.
Fig 3.
ELISA anti- Lutzomyia longipalpis saliva.
(A) O.D. from each non-human primate evaluated distributed according their families. (B) O.D. from non-human primates grouped in positive and negative to Leishmania spp. by xenodiagnosis. Positive animals had statistically significant higher O.D. values when compared to negative animals (*p = 0.0049 –Mann-Whitney test). Line represents the median and dots indicate individual values.
Table 3.
Phlebotomines captured within the area of the Zoological Garden in Belo Horizonte (Brazil), from February 2014 to February 2015.
Legend: # Pools: indicate the number of pools from each phlebotomine species that were prepared for DNA extraction.