Table 1.
Oncospheres of T. solium and T. saginata cultured in HCT-8 and INT-407 cells during 180 days.
Fig 1.
Light microscopy of postoncospheral form of T. solium and T. saginata displaying parasite morphology at different times of development in INT-407 culture.
T. solium and T. saginata at 15-day PO form (a) 30-day PO form (b) and at 60-day PO form (c) Magnification 100x.
Fig 2.
Light microphotography of T. saginata postoncospheral form at 120 and 180 days of culture in INT-407 cells.
At 120 days of culture a pre-scolex-like structure appears (a) and at 180 days of culture, a scolex appears (b). Magnification 100x.
Table 2.
Percent of rats infected after intracranial inoculation with T. solium and T. saginata oncosphere and PO form.
Fig 3.
Production of cytokines by human PBMCs stimulated with T. solium and T. saginata AO form.
Cultures supernatant were collected at 48 h from healthy human PBMC stimulated with 20 μg/ml of activated oncosphere antigens. (a) TH1- related cytokines, (b) TH2- related cytokines, and (c) other cytokines. Data presented are the mean ± SEM of healthy human donors (n = 10–13) minus control. * P< 0.05 was considered statistically significant.
Fig 4.
Production of cytokines by human PBMCs stimulated by T. solium and T. saginata PO 30-day forms.
Cultures supernatant were collected at 48 h from healthy human PBMC stimulated with 20 μg/ml of 30-day PO form antigens. (a) TH1- related cytokines and (b) TH2- related cytokines, and (c) other cytokines. Data presented are the mean ± SEM of healthy human donors (n = 10–13) minus control. * P< 0.05 was considered statistically significant.
Fig 5.
Production of MMP-9 by human PBMC stimulated with T. solium and T. saginata form.
Culture supernatant was collected at 48 h from healthy human PBMC stimulated with 20 μg/ml of activated oncosphere antigens and 20 μg/ml of 30-day PO form antigens of T. solium and T. saginata. Data presented are the mean ± SEM of healthy human donors (n = 5–6) minus control. * P< 0.05 was considered statistically significant; n.s. = not significant.