Table 1.
List of 15 references used in this study.
Table 2.
List of Leptospira isolates (n = 101) and Turneriella parva used for Leptospira MALDI-TOF MS database.
Table 3.
List of three MALDI-TOF MS training set for identification of biomarkers.
Fig 1.
MALDI-TOF protein mass spectra of 15 reference Leptospira spp.
Protein spectra of pathogenic group (red spectra), intermediate group (blue spectra) and non-pathogenic leptospires (green spectra) are presented. X and Y axis represent mass to charge (m/z) ratio and ion intensity, respectively.
Fig 2.
Dendrogram based on main spectrum profile (MSP) of 15 reference Leptospira spp.
The clustering based on MSP of pathogenic, intermediate and non-pathogenic group were determined.
Fig 3.
The protein mass spectra of L. interrogans at different bacterial concentration.
X-axis represent Spectra at mass to charge ratio of 2000–20000, Y axis is peak intensity at arbitrary unit (a.u.).
Table 4.
Identification score of blinded 97 Leptospira clinical isolates.
Table 5.
Peak statistics of 8 discriminating peaks for pathogenic leptospires included in the GA model.
Table 6.
Peak statistics of 8 discriminating peaks for intermediate leptospires included in the GA model.
Table 7.
Peaks for differentiating among 3 species of pathogenic Leptospira (L. interrogens, L. kirschneri, and L. borgpetersenii).
Table 8.
Peaks for differentiating among 8 serovars of L. interrogans.
Fig 4.
The protein profile matching of spiked leptospires in urine and media.
The protein spectra of L. interrogans, L. borgpetersenii, L. wolffii and L. biflexa in culture media (a), spiked in urine (b). Urine without any leptospires used as control (c).
Fig 5.
Principle component analysis (PCA) of protein spectra from spiked leptospires in urine and media.
Each dot displayed a protein spectrum of each sample. Color dot represent L. interrogans (Li), L. borgpetersenii (Lb), L. wolffii (Lw) and L. biflexa (Lx) in media and urine. The clustering of pathogenic, intermediate and non-pathogenic group is demonstrated in dash line.