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Table 1.

Dam and litter demographics based on dam’s Leishmania diagnostic status.

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Table 2.

Univariate analysis of risk factors for vertical transmission from dam to pup.

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Table 3.

Dam ever dx positive and qPCR positive during year of pregnancy significantly associated with Leishmania transmission to pups.

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Fig 1.

Kaplan-Meier time to offspring diagnostic positive based on dam’s diagnostic status ever.

Proportion healthy refers to the proportion of dogs that were diagnostically negative via qPCR and ELISA and DPP CVL assay. Blue represents the diagnostic status of pups from dams who were diagnostically negative during their lifetime. Red represents the diagnostic status of pups from dams who were diagnostically positive at any point during their lifetime via qPCR or serology. Shaded area represents variance around the mean. (chi-squared: 26.28 p-value <0.0001).

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Fig 2.

Kaplan-Meier time to dog diagnostic positive based on dam’s qPCR diagnostic status during the year of pregnancy.

Proportion healthy refers to the proportion of dogs that are diagnostically negative via qPCR. Blue represents the diagnostic status of pups from dams who were diagnostically negative during year of pregnancy via qPCR. Red represents the diagnostic status of pups from dams who were diagnostically positive via qPCR during year of pregnancy. (chi-squared: 55.70 p-value <0.0001).

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Fig 2 Expand

Fig 3.

Kaplan-Meier time to dog diagnostic positive based on mother’s serological diagnostic status during pregnancy.

Proportion healthy refers to the proportion of dogs that are diagnostically negative via ELISA and DPP CVL assay. Blue represents the diagnostic status of pups from dams who were diagnostically negative during year of pregnancy via ELISA and/or DPP CVL assay. Red represents the diagnostic status of pups from dams who were diagnostically positive via ELISA and/or DPP CVL during year of pregnancy.

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