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Fig 1.

Geographical locations and histograms showing the number of bugs collected per infested houses at the three municipalities, departments and countries.

Inserted maps represent each location (A: Olopa, Chiquimula; B: Texistepeque, Santa Ana and C: San Marcos de la Sierra, Intibucá) and colored dots represent houses within each village or canton. Histograms show the number of insects collected per infested house and the histogram colors indicate the locations in the inserted maps. The maps were created by RL and LS, specifically for this publication using the software package R. We obtained shapefiles from the GADM database of Global Administrative areas (http://gadm.org/) downloaded July 24, 2017 and house coordinates from this study.

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Fig 1 Expand

Fig 2.

Study design.

Sequential components of the study (black boxes), data analysis and statistical test for each component (dark grey box), the number of specimens included in each component (light grey box) and results associated with each component (white boxes).

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Table 1.

Distribution of houses surveyed, entomological indices and total number of bugs collected and analyzed by PCR for the three localities surveyed in August-September 2011.

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Table 1 Expand

Table 2.

Distribution of potential blood sources and peridomestic risk factors for the three localities surveyed in August-September 2011.

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Table 2 Expand

Fig 3.

Blood sources and Trypanosoma cruzi infection summary for bugs analyzed from each location.

Percentage of Triatoma dimidiata positive or negative by PCR for blood sources and T. cruzi prevalence from the three localities: Texistepeque, Olopa, and San Marcos de la Sierra. Components II and III refer to Fig 2.

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Fig 4.

Percent of blood meals detection for each of the seven taxa assayed by PCR in T. dimidiata from the three locations (Fig 2, Component III).

Note percentages sum to slightly more than 100 because of multiple blood meals detected in a few bugs (see details in text and S1 Table). *Indicates values are significantly different (p < 0.001) relative to other localities.

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Table 3.

Odds ratios for the ecological and life history factors that showed statistically significant association with bug infection with T. cruzi at each locality.

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Table 3 Expand