Table 1.
Summary of filtered RNA-seq data for deltamethrin-resistant and -susceptible Aedes albopictus individuals.
Fig 1.
Gene Ontology (GO) analysis of differentially expressed genes (DEGs) of Aedes albopictus transcriptome annotated to UniGene cDNA reference data.
Analysis was performed with Blast2GO-PRO. A) Histogram of Ae. albopictus differentially expressed sequences GO classification. Three main ontologies of GO (biological process, cellular component, and molecular function) are shown in the x-axis. The left y-axis indicates the percentage of total genes, and the right y-axis is the number of genes in each category. B) Distribution of transcripts in category of cellular components. C) Distribution of transcripts in category of molecular functions. D) Distribution of transcripts in category of biological processes.
Table 2.
Differentially expressed genes involved in detoxification in deltamethrin resistance and susceptible Aedes albopictus.
Table 3.
Nucleotide polymorphism of genes belonging to metabolic P450 gene family and Glutathione-S-Transferase (GST) significantly expressed between deltamethrin-resistant and -susceptible Ae. albopictus individuals.
Fig 2.
Comparison of expression value measured by RNA-seq and qRT-PCR.
A) Fold change of expression value in the resistant individuals relative to the susceptible individuals, as measured by RNA-seq and qRT-PCR in 11 selected genes. B) Pearson correlation between fold changes in gene expression between resistant and susceptible mosquitoes, as determined by qRT-PCR and RNA-seq (P<0.05).
Table 4.
Effects of RNAi on the expression of three candidate resistance genes in Aedes albopictus.
Microinjection and oral delivery RNAi were used.
Table 5.
Association between selected P450 gene SNP frequency and deltamethrin resistance in natural Aedes albopictus populations.