Table 1.
List of RVF virus isolates analyzed in the study, with GenBank accession numbers assigned to the nucleotide sequences of their respective L, M and S segments.
With the exception of Madagascar, origin imply the South African Province and nearest town from which the isolate originated.
Fig 1.
Photograph of a representative agarose gel in which SISPA products of RVF viruses were resolved.
Lanes contain products as follows: lane 1: M03/10, lane 2: M15/10, lane 3: M06/10, lane 4: M19/10, lane 5: M21/10, lane 6: M22/10, lane 7: M23/10, lane 8: M25/10, lane 9: M26/10, lane 10: M33/10 lane 11: no DNA. Lanes labeled M contain DNA size markers, with corresponding sizes of some indicated in kilo basepairs (kb).
Fig 2.
A-C. Phylogenetic trees derived from nucleotide sequence data of the three genomic segments of RVF virus segments. Segment S (A), Segment M (B) and Segment L (C) follows the same Lineage names as described by Grobbelaar et al., 2013. All Lineages containing RVF viruses from South Africa are presented in green. The two segment M re-assortments are indicated in red.
Fig 3.
Welling antigenicity plots of proteins encoded by the M segments of isolates M33_RSA_10 in blue, ZH501-Egy-77 in black and M37_RSA_08 in green.
Differences in amino acids residues among the three isolates are indicated on top of the antigenicity plots, with each isolate represented in its assigned colour. A graphical representation of the Non-structural protein (NSm), and glycoproteins (Gn) and (Gc) regions separate the antigenicity plots from the graph depicting the proportion of substitutions per amino acid position. These are representative of the 23 sequences of the M segments analyzed in this study and those of the previously published RVF viruses listed in S1 Table.