Table 1.
The detection of vector-borne bacteria, Anaplasma, Ehrlichia, Rickettsia, Coxiella and Bartonella, in the spleen samples of 35 small wild mammals from Chongming Island, Shanghai city.
Fig 1.
Spleen microbiota compositions at phylum level.
Color-coded bar plot showing the proportion of different bacterial phyla present in 35 mouse and shrew samples from Chongming Island, Shanghai city. The 4 shrew samples were marked with *, and the left 31 samples were mice; Samples marked with + were Anaplasma-positive.
Fig 2.
Spleen microbiota compositions at genus level.
Color-coded bar plot showing the proportion of different bacterial genera present in 35 mouse and shrew samples from Chongming Island, Shanghai city. The 4 shrew samples were marked with *, and the left 31 samples were mice; Samples marked with + were Anaplasma-positive. Taxa with <1% abundance were listed as others.
Fig 3.
Heat map of spleen microbiota compositions at genus level.
Log-scaled percentages of the abundances for the top 50 taxa at genus level in 35 mouse and shrew samples from Chongming Island, Shanghai city were illustrated in the heat map. The color scale (log10%) was on the left.
Fig 4.
Diversities in the spleen microbiota of 35 mice and shrews.
Principal coordinate analysis (PCoA) plots of the spleen microbiota based on the Bray-Curtis metrics considering animal genders, types, geographic sites or infection with Anaplasma. (A), male versus female groups. (B), mouse versus shrew groups. (C), different geographic site groups. (D), Anaplasma-positive versus Anaplasma-negative groups. F: female; M: male. Mi: mice; Sh: shrews. A: Anaplasma-positive; N_A: Anaplasma-negative.
Fig 5.
Anaplasma-positive and Anaplasma-negative samples were hierarchically clustered using UPGMA algorithm based on Bray-Curtis distances. A: Anaplasma-positive, in red; N_A: Anaplasma-negative, in green.
Fig 6.
Differentially abundant bacterial taxa in the spleen microbiota between Anaplasma-positive and Anaplasma-negative samples identified by LEfSe analysis.
Cladogram showed the highly represented taxa in Anaplasma-positive and Anaplasma-negative samples, respectively. A: Anaplasma-positive, in red; N_A: Anaplasma-negative, in green.
Fig 7.
Histogram of the linear discriminant analysis (LDA) scores.
Differentially abundant bacterial features enriched in Anaplasma-positive and Anaplasma-negative samples, respectively. The absolute values of LDA scores were > 2 (p < 0.05). A: Anaplasma-positive, in red; N_A: Anaplasma-negative, in green.
Fig 8.
Differentially abundant phyla enriched in Anaplasma-positive and Anaplasma-negative samples, respectively, analyzed by Wilcoxon rank-sum test (p < 0.05; q< 0.01). A: Anaplasma-positive, in green; N_A: Anaplasma-negative, in yellow.