Fig 1.
Standards for Reporting of Diagnostic Accuracy (STARD) flow chart of the 150 suspected leptospirosis cases enrolled in the study.
Patients were selected in two Brazilian cities: Curitiba (A) and Salvador (B).
Table 1.
Precision analysis of the lipL32 real-time PCR assay.
Fig 2.
Effect of different matrices on the accuracy of the lipL32 real-time PCR test.
Microscopically counted leptospires were used to spike EWB, serum and ultrapure water to 106 leptospires/mL. Samples were diluted ten-fold serially and the respective DNA extracts were quantified by real-time PCR. Each point represents the mean result of four spiking experiments performed on water, whole blood and serum. Error bars show the mean ± SD.
Fig 3.
Positivity of the lipL32 real-time PCR assay according to days after onset of symptoms in suspected cases of leptospirosis.
The bars represent the percentage of positive whole blood samples stratified according to days with symptoms (error bars show the 95% CI). The dashed line shows the geometric mean of the reciprocal MAT titer, in accordance with days with symptoms.
Table 2.
Characteristics of confirmed cases of leptospirosis.
Patients were identified during active surveillance in two Brazilian cities (Salvador and Curitiba), obtained on hospital admission.
Table 3.
Diagnostic performance of the real-time PCR detection assay for confirmed and unconfirmed cases of leptospirosis and control subjects.
Table 4.
Real-time PCR assay results according to leptospirosis clinical outcome and sample type.