Table 1.
Demographic and clinical characteristics of patients.
Fig 1.
Phylogenetic reconstruction of HSP70 sequences.
Phylogenetic reconstruction of 150 HSP70 gene sequences obtained from direct samples of CL patients and the reference strain retrieved from GenBank. L. braziliensis (green); L. panamensis (yellow); L. mexicana (black) and L. lainsoni (red). Futhermore, different genotypes associated with L. braziliensis (light green) and L. panamensis (light pink) were identified. The numbers represent the coding of each sample.
Fig 2.
Relationship between clinical-epidemiological data and infecting Leishmania species.
The frequency of linkages between species of Leishmania and the specific characteristics were divided into three levels: 1. Thickness lines,> 35 links, 2. Intermediate thickness lines, 15–35 links and 3. Semi-dotted lines of weak thickness, <15 links. The links with L. braziliensis were highlighted in red because of the high frequency of identified cases. “no”: it refers to absence of clinical manifestation.
Table 2.
Relationship between the clinico-epidemiology features and the infectious Leishmania species.
Table 3.
Genetic diversity parameters of Leishmania Cytb and HSP70 genes sequences.
Fig 3.
Geographical distribution of 222 Leishmania isolates associated to CL in Colombia.
GPS coordinates were used to build georeferenced maps of isolates location. The maps were built on ArcGIS10.3 using Esri Colombia PublicadorSIG layer. (http://www.arcgis.com/home/item.html?id=b051fbef7fba406fbb8e62b90925f365#overview) (A) Georeferenced isolates discriminated by species in the country. (B) Relative abundance of Leishmania species in each Colombian department. The size of the circle refers to the number of samples collected by department.
Fig 4.
Network analysis of geographical distribution of Leishmania species.
Alleles of the HSP70 gene were retrieved to construct the networks shown for each species as follows, the numbers on the lines specify the positions across the alignment where a nucleotide change occurred (A) L. braziliensis and (B) L. panamensis.