Fig 1.
Sex ratio of Schistosoma mansoni (male parasites / female parasites) in function of parasite load.
The parasite load was measured in 18 infected control mice and 57 mice infected with 150 exposed cercariae per mouse. (A) Parasite load in control group: linear regression R2 = 0.85, Y = 4.0 E-3x + 87.13; (B) Parasite load in Swiss Webster mouse infected with latex-exposed S. mansoni: linear regression R2 = 0.002, Y = 5.2 E-5x - 1.7752. Every point represents perfusion of worms from all mice of single cage (5-20 mice). The cages were also used for Kato-Katz.
Table 1.
Effect of exposure to Euphorbia milii latex on the adult parasite fitness parameters.
Control group was composed with of 18 mice infected with 150 mock treated cercariae per mouse and exposed group was composed of 57 mice infected with 150 exposed cercariae per mouse. All infections were performed by standard percutaneous inoculation and mixed sexes. Comparisons between groups were done by Student´s t-test.
Fig 2.
Hepatic granuloma of mice infected with Schistosoma mansoni.
The samples were randomly chosen among 18 mice from control group and 57 mice from exposed group. (A) Hepatic granulomas in control group. (B): Hepatic granulomas in Swiss Webster mouse infected with latex-exposed S. mansoni. Bar: 200 μm.
Fig 3.
Scanning electron micrographs of adult male Schistosoma mansoni.
Oral and ventral suckers of parasites from control (A) and latex-exposed groups (B); Tegument of worms from control (C) and latex-exposed groups (D); Gynecophoral channel of worms with spines from control (E) and without spines for latex-exposed ones (F).
Fig 4.
Scanning electron micrographs of adult female Schistosoma mansoni.
Oral and ventral suckers of female parasites from control (A) and latex-exposed groups (B).
Fig 5.
2D gels of adult females (left) and males (right) of Schistosoma mansoni.
For each sex, spots over-expressed in latex-exposed group as compared to control group are indicated in red while under-expressed ones are represented in green. Protein identification of spots by MS/MS is available in S3 Table.
Fig 6.
Pathways affected in Schistosoma mansoni adult worms after exposure to latex at cercarial stage.
Anabolic and catabolic pathways affected only in male (left, blue), only in female (right, red) or in both sexes (middle, purple). Pathways down-expressed are circled by a dotted lines and pathways over-expressed are circled with full lines.