Table 1.
Clinical profiles of 119 cutaneous leishmaniasis patients.
Fig 1.
Schematic presentation of sampling lesions with tape strip discs, DNA isolation and further identification of Leishmania species.
1) patient´s lesion 2) disc 3) disc applied to patient´s lesion 4) even pressure applied on lesion using a plunger 5) sterile removal of disc from the lesion 6) DNA isolation from disc 7) confirmation of Leishmania by kDNA1 8) species-specific identification (PCR ITS-RFLP).
Fig 2.
Illustration of sample collection using tape strip disc.
A) determine the lesion for disc sampling B) apply the tape strip disc on the lesion C) apply even pressure for 20 seconds with the plunger D) detach the disc and transfer into a sterile vial for DNA isolation.
Fig 3.
A) eyelid B) around the eye C) ear D) on the lips.
Table 2.
Disease duration, gender distribution and infecting species of Leishmania in CL patients with acute, chronic or healed disease.
Fig 4.
Genomic DNA isolation from healthy and infected skin.
The concentration of DNA from healthy skin is lower (lanes 1–3, mean 1.9 ng/μl) as compared to infected skin (lanes 4–10, mean 19.5 ng/μl)
Fig 5.
Agarose gel electrophoresis of amplified ITS1 regions of standard reference strains.
H2O (lane 1) L. major (lane 2) and L. tropica (lane 3) and from CL patients (lanes 4–9) in panel A and restriction endonuclease HaeIII digestion is shown in panel B. In both panels, MW representative of 100-bp ladder as molecular size marker. Patients in samples 2, 3, 4, 6, 7 and 9 are infected with L. tropica and patients in samples 1, 5 and 8 are infected with L. major.
Table 3.
Diagnostic accuracy of parasite culture from biopsies and tape strip disc.