Table 1.
Details of gene targets for gene expression analysis.
Fig 1.
Relative expression of genes involved in immune response and epigenetic regulation in melioidosis patients compared to patients with sepsis infections caused by other pathogens and healthy controls.
Statistically significant differential expression of genes in PBMC’s from melioidosis patients (n = 30), septicaemic melioidosis patients (n = 18) compared to sepsis controls (n = 10). Significant differential expression was not observed among melioidosis patients compared to healthy controls (n = 10) while significant differential expression was observed when compared to sepsis controls. Expression levels were normalized against 18SrRNA. Relative expression ratio based on efficiency corrected delta delta Ct values presented as log values to the base 2, >1.5 considered as up regulation and ≤0.5 considered as down regulation, with P<0.05 considered statistically significant.
Table 2.
mRNA expression in PBMC’s of melioidosis patients (n = 30) compared to other sepsis cases (n = 10) and healthy negative controls (n = 10).
Fig 2.
Relative expression of genes involved in immune responses and epigenetic regulation in melioidosis patients compared to patients with sepsis infections caused by other pathogens, in relation to duration of fever/clinical symptoms and antibiotics treatment.
Differential gene expression in PBMC’s from melioidosis patients with ≤15 days of fever (n = 4), melioidosis patients with >15 days of fever (n = 25), melioidosis patients with ≤15 days of treatment with antibiotics (n = 15), melioidosis patients with >15 days of treatment with antibiotics (n = 12) compared to sepsis controls (n = 10), did not change significantly due to the duration of fever or duration of treatment with antibiotics. Expression levels were normalized against 18S rRNA. Relative expression ratio based on efficiency corrected delta delta Ct values presented as log values to the base 2, >1.5 considered up regulated and ≤0.5 considered as down regulation, with P<0.05 considered statistically significant.
Fig 3.
Relative expression of genes involved in immune responses and epigenetic regulation in melioidosis patients compared to patients with sepsis infections caused by other pathogens, in relation to associated comorbidities.
Differential gene expression in PBMC’s from melioidosis patients with underlying commorbidities/risk factors (n = 27), alcoholic melioidosis patients (n = 8), diabetic melioidosis patients (n = 20), compared to sepsis controls (n = 10), did not change significantly due to associated commorbidities/risk factors for melioidosis. Expression levels were normalized against 18S rRNA. Relative expression ratio based on efficiency corrected delta delta Ct values presented as log values to the base 2, >1.5 was considered as up regulated and ≤0.5 was considered as down regulated, with P<0.05 considered statistically significant.
Table 3.
mRNA expression in PBMC’s of septicaemic melioidosis patients (n = 18), melioidosis patients with underlying comorbidities/risk factors (n = 27), melioidosis with ≤15 days of fever (n = 4) compared to sepsis negative controls (n = 10).
Table 4.
mRNA expression in PBMC’s of melioidosis patients with >15 days of fever (n = 25), melioidosis patients with ≤15 days of antibiotics (n = 15), melioidosis patients with >15 days of antibiotics (n = 12) compared to other sepsis cases (n = 10).
Table 5.
mRNA expression in PBMC’s of melioidosis patients with diabetes (n = 20), melioidosis patients with regular alcohol consumption habits (n = 8) compared to healthy controls (n = 10) and sepsis controls (n = 10).