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Fig 1.

CONSORT diagram of enrollment and follow up of subjects for first and second dosings of TV003 in subjects who were flavivirus-exposed prior to vaccination with TV003 (Study CIR280).

The schedule of blood draws for serological and virological testing is indicated in the shaded box at right.

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Table 1.

Adverse events following first and second dose of TV003 in flavivirus-experienced individuals.

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Table 2.

Viremia following the first dose of TV003 in flavivirus-experienced subjects (n = 41)a.

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Fig 2.

Mean plaque reduction (50%) neutralizing titers (PRNT50) for DENV-1 (A), DENV-2 (B), DENV-3 (C), and DENV-4 (D) following one or two doses of TV003 in flavivirus-experienced subjects (n = 41 for Dose 1, and n = 33 for Dose 2). All data represent mean titers (GMT) ± 95% confidence interval. Dotted line indicates seropositivity (PRNT50 ≥ 1:10) and gray area represents the range of Ab levels to each serotype observed in subjects who received placebo (n = 17 for dose 1 and n = 15 for Dose 2).

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Table 3.

Mean peak neutralizing antibody titers to TV003 in subjects who were flavivirus-experienced or -naïvea prior to vaccination.

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Table 4.

Reciprocal mean neutralizing antibody titers 6 months after dose 1 (Day 180) and dose 2 (Day 360) after vaccination with TV003 of subjects who were either flavivirus-experienced or–naïvea at time of first vaccination.

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Fig 3.

Mean peak neutralizing antibody titers (PRNT50) for DENV-1(A), DENV-2 (B), DENV-3 (C), and DENV-4 (D) up to 90 days following one dose of TV003 in flavivirus-naïve subjects (n = 58, from ref. [19]), all flavivirus-experienced (all FV+) subjects (n = 38) and by two subgroups–those with yellow fever virus (YF+, n = 31) or dengue virus exposure (Dengue, n = 11). All data represent mean titers (GMT) ± 95% confidence interval. Statistical significance of the difference between FV-naïve group was assessed by one-way ANOVA with Sidak correction for multiple comparisons. *, P < 0.05, **, P < 0.01, *** P, < 0.001.

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Table 5.

Frequency of seropositivity to each DENV serotype after one or two doses of TV003 in subjects who were flavivirus-experienced or -naïvea prior to vaccination.

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Table 6.

Valency of DENV responses after one or two doses of TV003 in subjects who were flavivirus-experienced or -naïvea prior to vaccination.

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