Fig 1.
Antibodies to whole cell antigens of B. pseudomallei K96243 wild type and OPS mutant (ΔwbiD K96243).
ELISAs were performed on the plates coated with antigens using serum from melioidosis patients, Thai healthy donors and U.S. healthy donors at dilution of 1:2000. Box plots represent 25th and 75th percentile boundaries in the box with the median line within the box; the whiskers indicate the 10th and 90th percentiles. The plots show OD 450 nm for each antigen.
Table 1.
Results of ELISA for antibodies to Hcp1 and OPS.
The results were obtained from OD450 values of sera from melioidosis patients, Thai healthy donors, U.S. healthy donors, tuberculosis patients, scrub typhus patients and leptospirosis patients at dilution of 1:2000.
Fig 2.
Receiver Operating Characteristics (ROC) plots of the ELISAs of Hcp1, OPS and combined Hcp1/OPS with different groups of serum.
(A) Serum form melioidosis patients versus Thai healthy donors, (B) Serum form melioidosis patients versus U.S. healthy donors. The serum samples were diluted 1:2000 for all ELISAs.
Table 2.
Sensitivity and specificity of Hcp1-ELISA, OPS-ELISA and Hcp1/OPS-ELISA.
The assay values were calculated from Thai patients who had melioidosis, tuberculosis, scrub typhus, leptospirosis and Thai healthy donors. The cut-off values with Thai healthy donors as controls were used at specificity of 95% for each assay.
Fig 3.
Antibody titers to Hcp1 (A) and OPS (B) in diabetic and non-diabetic melioidosis patients.
ELISAs were performed on the plates coated with antigens using undiluted sera and two-fold serially diluted sera from patients. Box plots represent 25th and 75th percentile boundaries in the box with the median line within the box; the whiskers indicate the 10th and 90th percentiles. The plots show antibody titers for each group of patients. The antibody titer was determined by ELISA using cut-off titers at specificity of 95%.
Table 3.
Sensitivity of ELISA for antibodies to Hcp1 and OPS in different groups of melioidosis patients.
The results were obtained from OD450 values of sera from melioidosis patients at serum dilution of 1:2000. The cut-off values with Thai healthy donors as controls were used at specificity of 95% for each assay.
Fig 4.
Antibody titers to Hcp1 antigen (A) and OPS antigen (B) in melioidosis patients with bacteremia and non-bacteremia patients.
ELISAs were performed on the plates coated with antigens using undiluted sera and two-fold serially diluted sera from patients. Box plots represent 25th and 75th percentile boundaries in the box with the median line within the box; the whiskers indicate the 10th and 90th percentiles. The plots show antibody titers for each group of patients. The antibody titer was determined by ELISA using cut-off titers at specificity of 95%.
Fig 5.
Antibody titers to Hcp1 antigen (A) and OPS antigen (B) in melioidosis patients who survived or died.
ELISAs were performed on the plates coated with antigens using undiluted sera and two-fold serially diluted sera from patients. Box plots represent 25th and 75th percentile boundaries in the box with the median line within the box; the whiskers indicate the 10th and 90th percentiles. The plots show antibody titers for each group of patients. The antibody titer was determined by ELISA using cut-off titers at specificity of 95%.
Fig 6.
Longitudinal analysis of antibody titers for Hcp1 and OPS in serum samples of 103 melioidosis patients who were survived within one year.
The antibody titer was determined at week 0, week 12 and week 52 by ELISAs using cut-off titers at specificity 95%. (A) serum antibody titers, (B) percent of serum samples with increased, decreased and no change in antibody titers between week 0 and 12, between week 12 and week 52, and between week 0 and week 52.
Fig 7.
Scatterplots of individual antibody titers to Hcp1 and OPS in serum samples of 103 melioidosis patients who were survived within one year.
(A) Sera from all time points, (B) sera of week 0, (C) sera of week 12 and (D) sera of week 52. For each plot, the relationship between variables as determined by linear regression (dotted line), Spearman correlation (correlation coefficient, rho) and P-value are shown.