Fig 1.
Baseline antibody responses to PGL-I (A), LID-1 (B) and ND-O-LID (C) in reaction-free (n = 292) and reactional leprosy patients (RR+ENL, n = 160). The box shows the interval between the first and the third quartiles, the middle line represents the median. The p value refers to differences in OD medians. The traced horizontal line is the cut-off: PGL-I OD>0.25; LID-1 OD>0.3; ND-O-LID OD>0.923. The numbers above each box represent the positivity rate and the points above each box are outlier results. OD = optical density.
Fig 2.
Baseline antibody responses to PGL-I (A), LID-1 (B) and ND-O-LID (C) among reaction-free (n = 292), leprosy patients that developed RR (n = 119) and leprosy patients that developed ENL (n = 41). The box shows the interval between the first and the third quartiles, the middle line represents the median. The p value refers to differences in OD medians. The traced horizontal line is the cut-off: PGL-I OD>0.25; LID-1 OD>0.3; ND-O-LID OD>0.923. The numbers above each box represent the positivity rate and the points above each box are outliers results. OD = optical density; RR: reversal reaction; ENL: erythema nodosum leprosum.
Fig 3.
Group 1- BI negative patients: Antibody responses to PGL-I (A), LID-1 (B) and ND-O-LID (C) antigens in reaction-free patients (n = 196) and patients that developed RR (n = 23). The box shows the interval between the first and the third quartiles, the middle line represents the median. The p value refers to differences in medians of OD. The traced horizontal line is the cut-off value: PGL-I OD>0.25; LID-1: OD>0.3; ND-O-LID: OD>0.923. The numbers above each box represent the positivity rate and the points above each box are outlier results. OD = optical density; RR: reversal reaction.
Fig 4.
Group 2- Patients with 0<BI<3 (n = 72): antibody responses to PGL-I (A), LID-1 (B) and ND-O-LID (C) among reaction-free patients (n = 36) and patients that developed RR (n = 36). The box shows the interval between the first and the third quartiles, the middle line represents the median. The p value refers to differences in OD medians. The traced horizontal line is the cut-off: PGL-I OD>0.25; LID-1: OD>0.3; ND-O-LID: OD>0.923. The numbers above each box represent the positivity rate and the points above each box are outliers results. OD = optical density; RR: reversal reaction.
Fig 5.
Group 3- Patients with BI≥3 (n = 161): Baseline antibody responses to different M. leprae antigens.
Seropositivity to PGL-I (A), LID-1 (B) and ND-O-LID (C) antigens in patients that developed RR (n = 60), ENL (n = 41) and reaction-free (n = 60) patients. The box shows the interval between the first and the third quartiles, the middle line represents the median. The p value refers to differences in OD median. The traced horizontal line is the cut-off: PGL-I OD>0.25; LID-1: OD>0.3; ND-O-LID: OD>0.923. The numbers above each box represent the positivity rate and the points above each box are outliers results. OD = optical density; RR: reversal reaction; ENL: erythema nodosum leprosum.
Fig 6.
Receiver Operating Curve (ROC) for serology to (A) PGL-I, (B) LID-1 and (C) ND-O-LID antigens in leprosy patients who developed RR during follow-up and reaction-free.
Fig 7.
Receiver Operating Curve (ROC) for (A) PGL-I, (B) LID-1 and (C) ND-O-LID antigens in leprosy patients who developed ENL during follow-up and reaction-free.