Fig 1.
Map of head lice collection in the pygmy population from Congo-Brazzaville.
Table 1.
Oligonucleotide sequences of primers and probes used for real-time PCRs and conventional PCRs in this study.
Table 2.
Number of pygmy individuals infested with single or multiple clades of lice from Congo-Brazzaville.
Table 3.
Haplotype frequency of pygmies’ head lice per village in Congo-Brazzaville.
Fig 2.
Cytb haplotype networks of human body and head lice. Each circle indicates a unique haplotype and variations in circle size are proportional to haplotype frequencies. Pie colors and sizes in circles represent the continents and the number of their sequence for a haplotype.
The length of the links between nodes is proportional to mutational differences. Haplotypes identified in the present study are in bold.
Fig 3.
Maximum-likelihood phylogram of Pediculus humanus haplotypes based on partial 272-bp cytb gene with Pediculus schaeffi and Pthirus pubis as outgroups.
Fig 4.
Maximum-likelihood phylogenetic tree based on 340-bp fragment flaB gene of the Borrelia species.
Table 4.
Detection of head lice clades and pathogens in the pygmy population in Congo-Brazzaville.
Fig 5.
Maximum-likelihood phylogenetic tree based on 440-bp fragment rpoB gene of the Acinetobacter species and Moraxellaceae species, while Pseudomonas was used as an out group.