Table 1.
Description of samples tested in Chad and results obtained after FAT, RIDT and RT-qPCR on FTA papers.
All specimens correspond to original brain samples collected from dogs.
Fig 1.
(A) Results from NRC-R, with six positive results including the two middle cassettes (samples 93101 and 87006) demonstrating only a weak test line in the results window. (B) Results from Chad, with one negative result (sample 18/12) and four positive results. The upper line visible in the results window is the control (C) and the lower line represents the test (T). The presence of two lines depicts a positive result, whereas the presence of only the upper line indicates a negative result. The test is considered as invalid when the upper line is not visible (not shown).
Table 2.
Limit of detection of the RIDT using 8 different titrated rabies virus suspensions.
Table 3.
Intrinsic parameters of the RIDT after laboratory evaluation (NRC-R), under field conditions (IRED) and when combining both conditions (all).
Table 4.
Detection of viral RNA with RT-qPCR on Anigen test strip used in field conditions (IRED), in laboratory conditions (NRC-R) or combined.
Table 5.
Comparison results of the detection of viral RNA with RT-qPCR performed on FTA Whatman cards and Anigen test strips.
Table 6.
Evaluation of the RIDT Anigen test in NRC-R in an inter-laboratory trial.