Fig 1.
Morbidity of Japanese encephalitis in Zhejiang Province, China, 1952–2014.
Data from the National Notifiable Diseases Reporting System, a hospital-based passive reporting system. Laboratory testing method was established to examine reported cases since 2004 in Zhejiang Province.
Fig 2.
The counties of sample collection site in Zhejiang Province, China.
Maps were created by an online map service system (http://www.dituhui.com/).
Table 1.
Demographic characteristics of subjects.
Table 2.
Mosquitoes collected for Japanese encephalitis study in Zhejiang Province, China, 1982–3 and 2007–2014.
Table 3.
Distribution of neutralizing antibody against different Japanese encephalitis virus strains.
Fig 3.
Age-specific seropositive rates of Japanese encephalitis neutralizing antibody and average incidence rates in Zhejiang Province, 2010–2014.
Fig 4.
Geometric mean titers (A) and seropositive rates (B) of Japanese encephalitis neutralizing antibody on different year(s) post-booster dose.
Fig 5.
Phylogenetic tree on 1500-nt envelope gene of Japanese encephalitis virus strains.
The sequences of Zhejiang strains isolated in 1982–1983 and 2007–2014 in this study are marked in black rhombus and circle, respectively. Phylogenetic analysis was performed by the neighbor-joining method using the MEGA 6.2 software package (www.megasoftware.net). Bootstrap probabilities of each node were calculated using 1000 replicates. Murray Valley encephalitis virus strain MVEV-51 was used as an outgroup. Scale bars indicate the number of nucleotide substitutions per site.
Table 4.
Nucleotide and amino acid sequence similarities of envelope gene in selected Zhejiang Japanese encephalitis virus strains and vaccine strain (SA14-14-2).
Table 5.
Comparison of the amino acid residues of E protein between Zhejiang strains, SA14-14-2 and Beijing-1 strain.
Table 6.
Genotype distribution of Japanese encephalitis virus isolation in eastern China.