Table 1.
Fungal strains used in this study
Table 2.
Characteristics of Mp1p homologs in T. marneffei.
Fig 1.
Predicted domains of Mp1p homologs in T. marneffei.
Fig 2.
Phylogenetic analysis of the putative LBD of Mp1p homologs in T. marneffei and orthologs in other fungi.
Homologs of Mp1p in T. marneffei are shown in bold. The tree was constructed by maximum likelihood method with bootstrap values calculated from 1,000 trees and rooted on midpoint. The scale bar indicates the branch lengths that correspond to 0.5 substitutions per site as indicated. Names and accession numbers are given as cited in the GenBank database.
Fig 3.
Mp1p is a virulence factor of T. marneffei.
(A) Survival curves of Balb/c mice challenged with T. marneffei PM1, MP1 knockout mutant, MP1 complemented mutant and knockdown mutants (***P<0.0001). (B) Mean fungal burden in spleen, kidney, liver and lung of mice challenged with T. marneffei PM1, MP1 knockout mutant, MP1 complemented mutant and MP1 knockdown mutant at day 12 post-challenge (***P<0.001, **P<0.01, *P<0.05). Error bars represent standard deviations. (C) Histopathological examination of PAS stained internal organs of mice at day 12 post-challenge. T. marneffei yeast cells are shown in black arrows and tissue necrosis in blue arrows.
Fig 4.
Mp1p enhances T. marneffei survival in macrophages.
Intracellular survival of T. marneffei PM1, MP1 knockout mutant, MP1 complemented mutant and MP1 knockdown mutant in murine macrophages at 2, 8, 16, 24 and 48 hours post-inoculation (**P<0.01). Error bars represent standard deviations.
Fig 5.
Mp1p improves survival of Pichia pastoris in mice.
Fungal burden in spleens, livers, kidneys, and lungs of mice challenged with P. pastoris GS115 and GS115-MP1 at 24 hours post-challenge (*P<0.05, ***P<0.001). Error bars represent standard deviations.