Fig 1.
A: NS1 cassette. B: strip outside the cassette with the location of the 4 parts cut for the experiment, N: nitrocellulose, C: conjugate pad, S: sample pad, N1 and N2: nitrocellulose membrane. C: strip with the location of the whole S pad (WS) cut for the experiment.
Table 1.
Mean number of dengue RNA copies/μl recovered by extraction from the 4 parts of RDT strip compared to the direct extraction.
Fig 2.
Efficiency of dengue RNA extraction from different RDT parts (S, C, N1 and N2) compared to the direct extraction for all isolate dilutions for the four dengue serotypes.
In y axis: RNA recovery in percentage: (RDT/Dir)*100 = ratio of the mean number of dengue RNA copies/μl recovered by RDT extraction (S, C, N1 or N2 parts) over the mean number of dengue RNA copies/μl recovered by the direct extraction. X-axis: RSD = relative standard deviation for the different extraction techniques.
Table 2.
Mean number of dengue RNA copies/μl recovered by extraction from RDT S, WS parts and filter paper, compared to direct extraction.
Fig 3.
Efficiency of dengue RNA extraction from RDT-S part (7mm), RDT-WS part (15 mm) and FP (2 disc of 6mm) compared to the direct extraction for all isolate dilutions for the four dengue serotypes.
In y axis: RNA recovery in percentage: (RDTor FP/Dir)*100 = ratio of the mean number of dengue RNA copies/μl recovered by RDT(S part or WS: whole S part) or FP extraction over the mean number of dengue RNA copies/μl recovered by the direct extraction. X axis: RSD = relative standard deviation for the different extraction techniques.
Table 3.
Number of patients found positive by DENV.
All RT-PCR performed after extraction from RDT or filter paper and from neat serum for 99 patients admitted at Mahosot Hospital.
Table 4.
Comparison of agreements of the different techniques for RNA preparation with the neat serum extraction for Mahosot Hospital samples.
Table 5.
Number of patients positive by DENV.
All RT-PCR performed after extraction from RDT or filter paper and from neat serum for 362 patients admitted at Salavan Provincial Hospital.
Table 6.
Comparison of agreements of the different techniques for RNA preparation with the neat serum extraction for Salavan Provincial Hospital sample.