Table 1.
Sample collection of selected organs and excretions analyzed by qPCR for the Lfb1 gene, micro-agglutination test and histology.
Fig 1.
Antibody response in infected rats.
Rat anti-Leptospira antibody responses were measured by the micro-agglutination test. The box plot shows the log10 titers of the sera samples of eight rats from intraperitoneal (A), mucosal (B) and subcutaneous (C) groups (tested in triplicate at a starting dilution of 1:50). Leptospiral antibody levels in each group were only significantly different on day 7 (p-value < 0.05).
Table 2.
Results of blood, urine, feces and saliva samples from each infected group, analyzed by qPCR for the Lfb1 gene on days 1 to 25.
Table 3.
Renal colonization and urinary excretions of each infected group on day 30.