Table 1.
Summary of DENV testing by TaqMan in plasma, cellular component of whole blood, serum and clot specimens from blood donors from Puerto Rico, 2012–2013, n = 165, and negative controls from the contiguous U.S., n = 16.
Fig 1.
Detection of DENV RNA in blood components.
A) DENV-1 and DENV-4 average viral load observed in plasma (PL), the cellular component of whole blood (CCWB), serum (SE) and homogenized clot specimens (CL). DENV viral load average in each sample co-component is expressed in log10 PDU/mL. B) Ct value averages for DENV-1 and DENV-4 plasma (PL) and cellular component of whole blood (CCWB). Samples were collected from infected Puerto Rican blood donors, 2012–2013. Only samples with Ct values ≤38 were included in the quantitative analysis. Whiskers represent 10-90th percentile. Outliers are shown as black dots. Mean is shown as a “+” sign and median as a horizontal line inside the box. Statistical significance is indicated as follows: *p<0.001 vs CCWB; **p<0.05 vs CL; ***p<0.001vs SE; ****p<0.05 vs CL.
Fig 2.
Mean DENV-1 and DENV-4 viral loads in blood components.
A) DENV-1 and B) DENV-4 viral load averages observed in plasma (PL) (n = 29), the cellular component of whole blood (CCWB) (n = 29), serum (SE) (n = 21) and clots (CL) (n = 21) from samples from infected Puerto Rican blood donors, 2012–2013. Whiskers represent 10-90th percentile. Outliers are shown as black dots. Mean is shown as a “+” sign and median as a horizontal line inside the box.
Fig 3.
Linked comparison of DENV viral loads in blood components including clots.
A) DENV-1 and DENV-4 combined viral loads in plasma (PL), the cellular component of whole blood (CCWB) and clot (CL), n = 29 samples. B) DENV-1 and DENV-4 combined viral loads in plasma (PL), the cellular component of whole blood (CCWB) and fresher clot (CL), n = 6 samples.
Fig 4.
Culture of DENV-positive blood components.
A) Percentage of TaqMan-reactive cell culture supernatant from mosquito C6/36 cells infected with plasma (PL), the cellular component of whole blood (CCWB), serum (SE) or homogenized clots (CL) from samples from Puerto Rican blood donors, 2012–2013. B) Infectivity titers of DENV-1-positive PL or CCWB from samples from infected Puerto Rican blood donors, 2012–2013, in supernatants from a single passage in cultured mosquito C6/36 cells. C) Infectivity titers of DENV-4-positive PL or CCWB from samples from infected Puerto Rican blood donors, 2012–2013, in supernatants from a single passage in cultured mosquito C6/36 cells. For both B) and C), whiskers represent 10-90th percentile. Outliers are shown as black dots. Mean is shown as a “+” sign and median as a horizontal line inside the box.