Table 1.
Development of Taenia solium postoncospheral forms at two week of incubation using different cells lines.
Table 2.
Taenia solium postoncospheral forms size at two week of incubation using transwell system.
Fig 1.
Light micrographs of development of Taenia solium postoncospheral form in HCT-8 cell monolayer.
(a) Left: T. solium activated oncosphere at 1 day of culture viewed at 10x objective; Right: oncosphere with typical hooks. (b) Left: T. solium postoncospheral forms at 15 day of culture viewed at 10x objective; Right: nucleus and cells were present in small postoncospheral forms. (c) Left: T. solium postoncospheral forms at 30 days of culture viewed at 10x objective; Right: protuberance was observed in one end of the body. (d) T. solium postoncospheral forms at 60 days of culture viewed at 10x objective; structure like a belly button observed in the middle of the body.
Fig 2.
Fluorescence microscopy of T. solium postoncospheral forms stained with DAPI stain.
Number of nuclei in T. solium activated oncosphere (a) compared with the number of nuclei in T. solium postoncospheral forms at 15 (b), 30 (c) and 60 (d) days of culture.
Fig 3.
Scanning electron micrographs of T. solium postoncospheral forms.
(a) T. solium activated oncosphere showing elongated microvilli. (b) 15 day postoncospheral forms showing the protuberance at one end of the body. (c) Close-up of the tegument of 30 day postoncospheral form showing microtriches. (d) 30 day postoncospheral form showing vesicles.
Fig 4.
Scanning electron micrographs of the protuberance forming in T. solium postoncospheral forms at three growth points.
(a) Microtriches and (b) vesicles (arrows) observed in the surface of the protuberance at one end of the body at 15 (I), 30 (II) and 60 (III) days PO forms.
Fig 5.
Morphology of T. solium 30 day postoncospheral form.
(a) Haematoxylin stain of 30 day postoncospheral form showing a large central cavity and the tegument. (b) Transmission electron microscopy of the tegument of 30 day postoncospheral form showing microtriches and cells.
Fig 6.
Rat brain infected with T. solium postoncospheral forms.
(a) Rat brain 4 months post infection showing two extraparenchymal cysticerci (arrows). (b) Coronal sections of the brain containing an intraparenchymal cysticercus stained with Masson’s Trichrome staining showing tegument and scolex.
Fig 7.
Western blot of T. solium antigens at different forms.
(a) Using rabbit anti-oncosphere sera. (b) Using rabbit anti-cysticerci sera.