Table 1.
Primers for amplification and sequencing of the Pvmdr1 gene and four flanking polymorphic repeat regions.
Fig 1.
The Pvmdr1 gene and the relative position of the chosen MS.
The identified SNPs within Pvmdr1 and the two chosen MS downstream of the Pvmdr1 gene; m9.5 (9,489 bp downstream of Pvmdr1), m10.1 (10,120 bp downstream) and the two upstream MS: m10.4 (10,420 upstream) and m43.1 (43,168 bp upstream) are shown.
Fig 2.
Pvmdr1 polymorphism in Plasmodium vivax samples collected at 8 geographical sites.
Four Pvmdr1 alleles were detected in the study abbreviated as TYF, MYF, MYL and MFL. The TYF wild-type is shown in blue, MYF (single mutant, T958M) in yellow, MYL (double mutant, T958M+F1076L) in green, and MFL (triple mutant T958M+Y976F+F1076L) in red. The number of positive samples from each site is shown on the map in brackets, and the number of alleles and percentage frequency overall are shown in the legend.
Table 2.
Allelic diversity measured by expected heterozygosity (He).
Table 3.
Plasmodium vivax mdr1 alleles TYF, MYF and MYL and flanking microsatellite alleles.
Fig 3.
Genetic diversity of the Plasmodium vivax mdr1 gene in P. vivax samples collected at 9 malaria endemic districts of Sri Lanka.
Three different genotypes were detected in the samples from Sri Lanka named MYF(yellow), MYL(green) and MFL(red). MYF (single mutant, T958M), MYL (double mutant, T958M and F1076L) and MFL (triple mutant). The number of positive samples from each district is mentioned in brackets.
Fig 4.
Plasmodium vivax mdr1 combined with flanking microsatellite variation in P. vivax samples collected at 9 malaria endemic districts of Sri Lanka.
The size of the pie charts reflects the sample size, while the segments illustrate the frequency of combined Pvmdr SNP+ microsatellite haplotypes. Grey color segment indicates the proportion of the sample which consisted of unique 'singleton' haplotypes while the other colors indicate the proportion of each repeated haplotype. The black lines indicate sharing of a specific haplotype between two sites and multiple lines indicate the number of haplotypes shared between given sites.