Fig 1.
Anatomy of adult female B. malayi.
Tissues and structures dissected for proteomic analysis include body wall (red labels), reproductive tract [blue labels], and digestive tract (green labels). Illustrated by Wensi Sheng.
Fig 2.
Dissection process of adult female B. malayi.
Top left and bottom left show break in the body wall and extrusion of digestive and reproductive tracts. Top right and bottom right: Body wall is in process of being slid away from digestive and reproductive tracts. Magnification: top left: 40x, bottom left: 100x, top right: 30x, bottom right: 20x.
Fig 3.
Venn diagram of proteins identified within each anatomic fraction of adult female Brugia malayi based on 2 peptide minimum for identification.
Fig 4.
Association of transporter proteins with the digestive tract as measured by gene set enrichment analysis (GSEA).
P-value = 0.005. The enrichment score is represented by the green line. Proteins were rank ordered according to their number of spectral counts within the digestive tract and are depicted in the heat map (red = more abundant, blue = less abundant). Black vertical lines represent each of the proteins associated with transporter function. D = Digestive tract, R = Reproductive tract, and B = Body wall.
Table 1.
20 most abundant proteins, with proper names, enriched in the digestive tract of adult female B. malayi based on normalized spectral abundance factor (NSAF).
Table 2.
20 Most abundant proteins, with proper names, enriched in the body wall of adult female Brugia malayi.
Table 3.
20 Most abundant proteins, with proper names, enriched in the reproductive tract of adult female Brugia malayi.
Table 4.
Selected proteins from S1 Table that contain 1–2 transmembrane domains, a significant non-cytoplasmic portion, >75% homology to either W. bancrofti, or O. volvulus, and <40% homology to humans.