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Table 1.

Group and total number of Aelurostrongylus abstrusus and Troglostrongylus brevior larvae detected in the mucus of Helix aspersa snails or in the water solution where gastropods had died.

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Table 2.

Total number and percentage of Aelurostrongylus abstrusus and Troglostrongylus brevior L3 detected in each snail specimens from different groups following water flooding and digestion examinations.

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Fig 1.

Infective L3 of T. brevior.

(A) and A. abstrusus (B) detected in the mucus of H. aspersa snails at 25 days post-infection (scale bar = 50μm).

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Fig 2.

Histopathology: Free larvae of A. abstrusus (21 dpi) in snail foot observed in transverse (hash) and oblique sections (star) in the fibro-muscular tissues.

(A); oblique larval section of T. brevior (9 dpi) the subpallial tissue (arrow) (B) (scale bar = 50μm; H&E).

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Fig 3.

Histopathology: Inflammatory response to A. abstrusus in the H. aspersa.

Cell-poor granuloma formation with vacuolated amebocytes at 3dpi. (A); cell-rich granuloma formation at 9 dpi (B); small necrotic granuloma at 15 dpi (C); fibroblast-like reaction at 27 dpi (D) (scale bar = 50μm; H&E).

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Fig 4.

Histopathology: Larvae of A. abstrusus (asterisks) in the snail foot at 9 dpi, within granuloma formations.

(A); large necrotic granuloma with peripheral well-preserved amebocytes and their debris in the centre in snail infected by T. brevior at 21 dpi (B); polipoid enlargement of the ventral surface of the foot with dilated vessels and an abscess-like formation in T. brevior-infected snail (21 dpi) (C); multifocally-distributed amebocytes aggregates in the kidney parenchyma in T. brevior-infected snail (3 dpi) (D) (scale bar = 50μm; H&E).

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