Table 1.
Histopathological changes measured in this study.
Table 2.
Primer sequences and amplicon details for porcine qPCR.
Fig 1.
Clinical appearance of crusted (A) & ordinary scabies (B) at week 12 post infestation with Sarcoptes scabiei.
Table 3.
Approximate Sarcoptes scabiei numbers in skin scrapings collected from trial pigs.a
Fig 2.
Representative histology and immunohistochemistry of skin lesions at week 12 post infestation with Sarcoptes scabiei.
(A) Crusted scabies, hematoxylin and eosin stain; (B) Ordinary scabies, hematoxylin and eosin stain; (C) Crusted scabies, anti-CD3+ antibody T cell stain; (D) Crusted scabies, toluidine blue mast cell stain. Scale bars = 100μM.
Table 4.
Summary scores of histological changes in representative mange infested pigs.a
Fig 3.
Numbers of CD3+ cells (A) and mast cells (B) in skin biopsies collected from representative pigs at week 0, 4, 8 and 12 post-infestation with Sarcoptes scabiei.
Group A n = 2, B+ n = 2, B n = 2, C n = 2, D n = 2. Group B+ = pigs in group B that developed crusted scabies based on mite counts and lesion scores.
Fig 4.
Representative IL-17 staining of skin lesion at week 12 post infestation with Sarcoptes scabiei.
(A) Crusted scabies, (B) Ordinary scabies, (C) Non-infested, (D) anti-interleukin-17 isotype control antibody. Scale bars = 100μM.
Fig 5.
Changes in cytokine transcription in skin biopsies collected from all pigs at weeks 4, 8 and 12 post-infestation with Sarcoptes scabiei.
Group A n = 6, B+ n = 2, B n = 4, C n = 6. qRT-PCR was performed on 1μg total RNA and transcription normalised to the housekeeping gene HPRT1. Relative fold-changes in gene transcription in treatment groups were calculated by comparison to control pigs in Group D (n = 6). * = p<0.05, ** = p<0.001, ***p<0.0001 vs control, unpaired T test. Bars represent mean +/- SEM. Abbreviations: IL, interleukin, IFN, interferon, TGF, transforming growth factor.