Table 1.
Taqman primers and probe for detection of Leishmania DNA in endemic healthy controls.
Figure 1.
Flow chart of study of serology in endemic healthy control individuals (EHC) from baseline serosurvey (year 1) to identification of progressors.
SP – seropositive; SN – seronegative; QP – qPCR positive (CT cutoff 39); QN – qPCR negative; VL – progressors to symptomatic visceral leishmaniasis.
Figure 2.
Bar graph to show Leishmania detected by qPCR in different group of EHC on the basis of their serological status (QP-qPCR positive, QN-qPCR negative, SP-sero positive, SN-sero negative).
Table 2.
Parasitemia range in blood buffy coat samples from healthy controls from endemic or nonendemic regions.
Table 3.
Pogressors (disease conversion, VL cases) details: Parasite load and serostatus of EHC who were healthy at the first survey, but developed symptomatic VL at the time of the 12-month follow up.