Figure 1.
Satellite imagery (∼1 m resolution, ArcGIS, Ver. 10.1, ESRI Inc., Redlands, California, USA [26]) centered on the riverbed of Rio Chico near the town of Zurima (left).
Digitized town boundary (black polygon), riverbed (blue lines), six isolated houses (small black dots) and numbered locations of the positive (red fill) and negative (white fill) trap sites.
Figure 2.
Noireau sylvatic insect vector trap.
Traps consisted of plastic containers with a live mouse and wire mesh tops. Double sided sticky tape was placed on the outside of the traps.
Table 1.
Sequence of mitochondrial DNA primers (F = Forward, R = Reverse) and probes (P) used in cloning and qPCR assays to detect vertebrate blood meal sources in Chagas disease insect vectors.
Table 2.
Blood meal sources of the Chagas disease vector, Triatoma gusayana collected from sylvatic locations in Bolivia.
Table 3.
Match between primers used in the cloning assays to identify blood meal sources of Triatoma guasayana and the taxa detected, showing that the Melton primers are a better match than the Kitano primers.
Figure 3.
Fluorescence as a function of reaction cycles for (A) chicken, (B) Canis sp. and (C) human.
Positive controls were run in triplicate starting with 100 on the left (black) followed by each successive 10-fold serial dilution in a different color. Amplification curves are shown for the dilutions above the 10−4 limit at which template concentration that can be detected with 95% certainty.
Figure 4.
Chicken qPCR amplification of 14 samples (green), 5 serial dilutions (red) and NTC (no template control, black).
NTC are not visible because they did not amplify and rise above the X-axis.
Figure 5.
Canis sp. qPCR amplification of 14 samples (green), 5 serial dilutions (red) and NTC (no template control, black).
NTC are not visible because they did not amplify and rise above the X-axis.
Figure 6.
Human qPCR amplification of 14 samples (green), 5 serial dilutions (red) and NTC (no template control, black).
Figure 7.
Cp (crossing point) vs. serial dilutions for chicken (black circle), Canis sp. (blue square) and human (red triangle) assays in triplicate.